Active Antitoxic Immunization against Ricin Using Synthetic Peptides
Annual rept. 9 Mar 1988-8 Jul 1989
SRI INTERNATIONAL MENLO PARK CA
Pagination or Media Count:
This year, peptides with overlapping sequences of candidate A-chain peptides, i.e., 17-31, 84-100, 102-118, 175-191, and 227-243 were synthesized and conjugated to BSA. Immunization studies have been performed on most of the A- and B-chain peptides synthesized for the project. Antisera from the peptides were tested by ELISA for their binding to corresponding immobilized peptides corresponding ricin subunit, and intact ricin. Challenge studies were performed on peptides eliciting cross-reacting antibodies. Although some peptides elicited high-titer, cross-reacting antibodies, none of them were able to provide protection against ricin challenge. Keywords Ricinus communis Plant toxins Ricin, Synthetic peptides, Immunization, RA 1 Antitoxins Active sites Binding sites cytoplasm.
- Genetic Engineering and Molecular Biology