Accession Number:

ADB134651

Title:

Sulfur Mustard (SM) Lesions in Organ-Cultured Human Skin: Markers of Injury and Inflammatory Mediators

Descriptive Note:

Annual rept. 17 Feb 1988-16 Feb 1989

Corporate Author:

JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF HYGIENE AND PUBLIC HEALTH

Personal Author(s):

Report Date:

1989-03-01

Pagination or Media Count:

41.0

Abstract:

In this research program, we are a developing in vitro human skin model with which to assess the amount of injury produced by topically applied sulfur mustard SM, and b identifying markers of cell death and early mediators of the inflammatory response produced by SM in human skin. This second Annual Report will cover our progress since our first Annual Report, i.e., from February 17, 1988 to February 16, 1989. The most promising measure of skin injury is our paranuclear vacuolization test. Keywords Sulfur mustard Human skin lesions Histology histochemistry Inflammatory mediators Organ culture Viability of human skin at 4 C Paranuclear vacuolization test for epidermal injury Acid phosphatase Trypsin-like enzymes Deoxyribonuclease Ribonuclease Hydroxyproline Leukotriene B4 Interleukin 1.

Subject Categories:

  • Test Facilities, Equipment and Methods
  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE