Crotoxin: Structural Studies, Mechanism of Action and Cloning of its Gene
Annual rept. 1 Mar 1987-29 Feb 1988
WYOMING UNIV LARAMIE DEPT OF MOLECULARBIOLOGY
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The purpose of this project is to 1 gain greater insight into crotoxin and crotoxin homolog structure, in order to provide a better understanding of this class of rattlesnake neurotoxin 2 develop an in vitro system for examining presynaptic neurotoxin mechanism of action 3 clone the crotoxin gene as a first step in creating a non-toxic, but immunoreactive crotoxin analog and 4 explore other possible non-toxic, crotoxin immunogens as potential vaccines against crotoxin and its homologs. We have completed the sequence determination of both the basic and acidic subunits of crotoxin. Four presynaptic neurotoxins and their subunits have been examined using circular dichroism, deconvolution Fourier-transform infrared, and fluorescence spectroscopy. Results indicate a large conformational change occurs upon complex formation between the acidic and basic subunits of all four toxins. Keywords Crotoxin, Presynaptic neurotoxins, Rattlesnake toxins, Natural toxins, Antitoxins.