Development of Analytical Methods for the Determination of Drug Substances in Biological Fluids.
Annual rept. 1 Sep 84-31 Aug 85,
CALIFORNIA UNIV SAN FRANCISCO SCHOOL OF PHARMACY
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Methods available to quantitate halofantrine, an antimalarial drug with demonstrated activity against Plasmodium falciparum, in blood and plasma are limited. We report the development of a simple and rapid HPLC assay for the analysis of 0.5 ml blood and plasma specimens. The method detection limit is 1 ngml for both halofantrine WR 171,669 and its putative metabolite WR 178,460. The methods principal techniques include sample cleanup by protein precipitation and by a column elution step, precautions against loss of drug because of absorption to glass, and fluorescence detection. The method proved adequate in tests of linearity, recovery, precision, and accuracy. Drug stability tests of specimens stored at -20C and 80C indicated the necessity of storage at 80C. Routine analysis of human and animal blood plasma samples were satisfactory performed. Keywords Halofantrine WR 171,669, Putative Metabolite WR 178,460 Blood Blood Plasma.
- Medicine and Medical Research