Cloning of Acetylcholinesterase Gene in a Microbial Vector.
Annual Progress rept. May 82-Apr 83,
CALIFORNIA UNIV SAN FRANCISCO
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Efforts made toward the end of cloning synthetic deoxyribonucleic acid DNA sequences coding for the enzyme acetylcholinesterase are described. The methods described include the isolation, purification, and analysis of messenger ribonucleic acid mRNA from the electric organ of Torpedo californica, a tissue which is highly enriched in acetylchloinesterase. Also described is the construction of three libraries of clones of E. coli bacteria containing the synthetic DNA sequences, and the screening that has been done to isolate a clone or clones containing sequences coding for the enzyme. The conclusion is made that the correct clones have not yet been isolated, although the evidence strongly indicates that at least one bank should contain the clones of interest.
- Anatomy and Physiology