Control of Hepatitis Virus Infections by New Methods
Technical Report,01 Jun 1982,31 Jan 1983
BAYLOR COLL OF MEDICINE HOUSTON TX HOUSTON United States
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During the past contract year, we have continued our investigations with an HBsAg synthetic peptide SP1 which contains amino acid residues 122-137 associated with the ayw subtype sequences of the major envelope polypeptide, P25. Several important immunochemical parameters associated with SP1 were established 1 Conjugation of SP1 to a tetanus toxoid TT carrier or aggregation of SP1 in micelle form yielded material that produced a good booster response in mice. In fact, similar titers were noted in mice injected with 2 doses of alum-precipitated material of either SP1 conjugated to TT or purified HBsAg 22-nm particles. 2 immunization of a chimpanzee with preexisting anti-HBs with 2 inoculations of SP1-TT in alum produced a significant booster antibody response. 3 SP1 contains a conformational a epitope and a sequential y epitope. We have also produced an anti-idiotype reagent in rabbits that recognizes a common idiotypic determinant on human anti-HBs molecules. Finally, we have demonstrated that mice injected with specifically purified antiidiotype produced to human anti-HBs produce immune cells that secrete antibody which reacts specifically with HGsAg. We are currently investigating the potential of this reagent for the specific modulation of the immune response.