Accession Number:

ADB097240

Title:

Immunity in Dengue

Descriptive Note:

Final rept. 1 Jun 1969-31 Dec 1982

Corporate Author:

HAWAII UNIV HONOLULU (MANOA CAMPUS) JOHN A BURNS SCHOOL OF MEDICINE

Personal Author(s):

Report Date:

1983-09-01

Pagination or Media Count:

56.0

Abstract:

Prototype dengue DEN 4 H-241 was received as original viremic human serum and passed once in a susceptible monkey and twice in Aedes albopictus replicated in primary dog kidney and African green monkey kidney cells and characterized at passages 7, 15, 30 and 50. Serial weekly passage of undiluted virus was carried to the 50th passage in both primary cell cultures. Parental DEN-4 phenotype included large plaque formation in LLC-QMK2 cells, plaque formation in GMK cells, cytopathic effect in LLC-MK2, growth in human monocyte cultures, growth at 39 C, consistent production of viremia in monkeys and short-incubation neurovirulence in mice. By 7 passages in both cell cultures, DEN-4 viruses exhibited reduced plaque-size in LLC-MK2, failure to plaque in GMK, to produce CPE in LLC-MK2, or to grow in human monocytes. Serial passage in PDK as opposed to GMK, resulted in a graduated loss of monkey virulence. PDK 50 virus did not replicate at 39 C and only 1 of 4 inoculated monkeys developed an antibody response.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE