DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click HERE
to register or log in.
Development of Analytical Methods for the Determination of Drug Substances in Biological Fluids.
Annual rept. 1 Sep 83-31 Aug 84,
CALIFORNIA UNIV SAN FRANCISCO SCHOOL OF PHARMACY
Pagination or Media Count:
A high performance liquid chromatographic HPLC procedure for the analysis of pyridostigmine in plasma has been developed. Only 0.5 ml of plasma is required for the analysis. The clean-up procedure involves a protein precipitation step and a column elution step prior to separation by HPLC. The assay is quite sensitive, with a detection limit of 2 ngml for pyridostigmine bromide in plasma. Assay precision was evaluated from blind samples in the range of 0 - 50 ngml and the difference was 8 to 12. Stability was also determined for pyridostigmine in plasma at -20 C and -80 C. The results showed no degradation for pyridostigmine at -80 C for up to two months. In a preliminary study with one human volunteer, the drug was detected up to 8 hours following oral doses of syrup solution of 0.4 to 0.9 mgkg. This assay is suitable for pharmacokinetic studies involving pyridostigmine in human subjects.
APPROVED FOR PUBLIC RELEASE