Togavirus-Specific Immune Effector Mechanisms.
Annual progress rept. 1 Jan-31 Dec 82,
MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE
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A panel of monoclonal MC antibodies against Sindbis virus SIN envelope glycoproteins E1 and E2 was used, in an ELISA, to define five topographically distinct antigenic sites on E1 and on one site E2. A single anti-E1 MC antibody had neutralizing NT activity in vitro MC antibodies to the other four E1 sites did not NT SIN even in the presence of complement C or anti-mouse immunoglobulins. However, antibodies to all five E1 sites were able to mediate complement C-dependent lysis of SIN-infected cells. A competitive inhibition-ELISA demonstrated that non-NT anti-E1 antibodies did not bind to intact virions but bound to cryptic antigenic determinants epitopes that could be exposed by disrupting virions with a nonionic detergent. MC antibodies against all five E1 sites were able to passively protect adult C57BL6J mice from an otherwise lethal intracerebral challenge with a neuroadapted variant of SIN. Competitive antibody-binding assays provided strong evidence that epitopes of at least one E1 site are shared among several alphaviruses, suggesting that an immunization scheme could be devised to induce anti-E1 antibodies that would cross-react with, and protect against, several alphaviruses. Originator-supplied keywords include Western equine encephalitis virus, idiotypes, anti-idiotypic antibodies , cell culture.
- Medicine and Medical Research