A Survey of Compounds from the Antiradiation Drug Development Program of the U.S. Army Medical Research and Development Command
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC
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Female mice of the Walter Reed Bagg Swiss or Inbred Charles River ICR strains, 5-6 weeks old and weighing 21-25g, were randomly assigned to treatment groups. An estimation of the toxicity of each compound to be tested for antiradiation activity, i.e., a determination of the approximate LD50 ALD 50, was obtained in separate experiments prior to irradiation. Toxicity estimations were based upon animal survival over a ten-day observation period following intraperitoneal or oral administration of each agent in graded doses using 10 mice at each dose level. Adverse symptomatology was noted and, in addition, each mouse was subjected to a post mortem examination. The highest dose of each compound administered for antiradiation testing was about two thirds of the ALD50 unless a lower dose was indicated by adverse symptomatology or pathologic changes the selected doses of each compound were administered 15- 30 minutes before exposure to lethal whole body irradiation. Mortality was tabulated for a 30-day period for each individual animal and all data computerized. Under our conditions, approximately 800r x-ray and 1000r Co-60 gamma irradiation were equally lethal LD-10030, all control animals dying between the tenth and twenty-first days following exposure.
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