Detection and Measurement of ANtigen-Antibody Reactions Using Fluorescence Polarization Techniques.
Final rept. 1 Oct 61-30 Sep 65,
CORDIS CORP MIAMI FL
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After the pioneering biochemical studies on fluorescence polarization by Perrin nearly forty years ago the phenomenon was reintroduced by Weber in 1952 and, a few years later, by Steiner, to measure absolute values of relaxation times for macromolecules in solution. It soon became evident that the possibility of a certain amount of free rotation between the fluorescent label and the macromolecule made the method subject to large possible errors. For this reason, primarily, fluorescence polarization fell again into disuse. However, about the same time, the work on the binding of dyes to macromolecules carried out by Laurence clearly pointed the way to an entirely different application which is the subject of this investigation. Keywords Antigen-antibody reactions, Detection and measurement, Fluorescence polarization, UA Reports, Biochemistry. jg
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