Mechanisms of Hemolysis.
CALIFORNIA UNIV SAN DIEGO LA JOLLA SCHOOL OF MEDICINE
Pagination or Media Count:
Following cell-wall interaction trauma, relatively new erythrocytes are immediately lysed. A much larger number are signicantly damaged and undergo later destruction in vivo, or autohemolysis in vitro. The mechanism of this damage includes an increase in the oxidative metabolic cycle, and selective lipid elution. Lysolecithin activity and lipid peroxidation do not appear to be important mechanisms. Erythrocyte damage by surface or wall interaction is accompanied by the release of free 2, 3 DPG to the plasma, in greater concentrations than normally exist within the cell, compared to hemoglobin. Similar findings occur after lysis due to shear stress and osmotic forces. Following incubation, 2,3 DPG is rapidly depleted in both control and damaged specimens, and the oxyhemoglobin dissociation curve is shifted to the left to the expected degree. Only after osmotic lysis is RBC ATP concentration substantially depleted, although free plasma ATP is demonstrable following both shear and osmotically induced injury.
- Medicine and Medical Research