Antimicrobial Decapeptide KSL-W Enhances Neutrophil Chemotaxis and Function
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
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Mammalian cationic antimicrobial peptides have received increased attention over the last decade, due to their prokaryotic selectivity and decreased risk of microbial resistance. Ina addition, antimicrobial peptides display differential biological effects on mammalian immune cell function, such as migration, adhesion, and modulation of respiratory burst, which makes them even more attractive as therapeutic agents. Synthetic combinatorial libraries provide a time-efficient and cost-effective source for these diverse molecules. The novel synthetic antimicrobial peptide, KSLW KKVVFWVKFK-NH2, has shown to display a broad spectrum of antimicrobial activity against Gram and Gram - bacteria, fungi and viruses. In this study, we evaluated the alternative biological activity of the decapeptide on neutrophil migration and function. KSLW was demonstrated to be chemotactic for5 neutrophils in micromolar amounts, and neutrophil treatment with KSLW, after q minute, resulted in significate increases in F-actin polymerization. KSLW was show to inhibit oxygen radical production in PMA- and LPS-simulated neutrophils. Future studies, to determine if KSLW regulates neutrophil phagocytosis, adhesion, and apoptosis, or examining the effect of KSLW on other mammalian cell types, such as cell populations of healing-impaired wounds, would provide significant insight for the potential strategies offered by antimicrobial peptides.