Accession Number:

ADA634961

Title:

Nucleotide Sequence of the Hantaan Virus S RNA Segment and Expression of Encoded Proteins

Descriptive Note:

Doctoral thesis

Corporate Author:

UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD

Personal Author(s):

Report Date:

1987-11-03

Pagination or Media Count:

243.0

Abstract:

The determination of the nucleotide sequence of the Hantaan virus HTV genome M RNA segment was begun to devise a strategy for expressing the encoded proteins in a eukaryotic expression system and to allow a comparison with other HTV RNA segments. Concurrently, the complete nucleotide sequence of the HTV S RNA segment was determined from three overlapping eDNA clones. The S RNA segment was 1695 nucleotides in length encoding two possible proteins in a unique coding strategy for members of the Bunyaviridae family. A large open reading frame ORF followed by a second smaller ORF was found in the viral complementary-sense RNA. Complementary sequences were found at the 3 and 5 termini of the RNA molecules which would allow formation of a stable stem-loop structure and the formation of the circular nucleocapsid. The M and S RNA segments contained 3 noncoding regions of similar length with 20 of the nucleotides conserved. Conserved sequences which could act as possible transcription termination signals in the 5 noncoding regions were also observed.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE