Accession Number:

ADA634960

Title:

Electron Microscopic Analysis of the Effects of Psoralen and Interferon on Replicative Intermediates Formed During Encephalomyocarditis Virus Infection of Mouse L Cells

Descriptive Note:

Doctoral thesis

Corporate Author:

UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD

Personal Author(s):

Report Date:

1987-08-24

Pagination or Media Count:

99.0

Abstract:

Demonstration of the existence of base-paired RNA in replication intermediates of murine encephalomyocarditis virus is important in understanding the mechanism of viral replication, interferon induction, and the role of double-stranded RNA-requiring enzymes in the mode of interferon action. The psoralen derivative 4-aminamethyl-4,5,8-tri-methylpsoralen was used to specifically modify possible base-paired regions of the replicative intermediate formed during encephalomyocarditis virus infection. Psoralen modified replicative intermediates were shown to be formed in-vitro and in intact cells. Psoralen-modified replicative intermediates were isolated and examined in the electron microscope. Electron microscopic analysis suggested that encephalomyocarditis replicative intermediates contain significant stretches of base-pairs in intact cells. Further, replicative intermediates from interferon-treated cells were significantly shorter than those from control cells. This result suggests that the interferon-treatment led to viral RNA degradation, possibly through production and action of 2, 5-oligoadenylate. This study provides direct evidence that replicative intermediates of encephalomyocarditis virus contain base-pairs in intact cells, and are degraded as a result of interferon treatment.

Subject Categories:

  • Biology
  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE