Accession Number:

ADA634873

Title:

Role of the Wasp Venom Peptide Mastoparan in the Study of Mechanisms Involved in Cell Death

Descriptive Note:

Doctoral thesis

Corporate Author:

UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD

Personal Author(s):

Report Date:

1989-08-23

Pagination or Media Count:

168.0

Abstract:

The effect of mastoparan on membrane function in Madin Darby Canine Kidney MOCK cells was studied. Mastoparan increased 3 H- inositol phosphates formation 56 p.001, 172 p.001, and 231 p.001 following treatment of 25, 75, and 100 ugjml mastoparan, respectively. Mastoparan-induced LDH release reached 8 at 1 min p.005 and 32 at 15 min p.001. Mastoparan also mediated the accumulation of inositol-trisphosphate with a concommitant decrease in the level of phosphatidylinositol-bisphosphate. Furthermore, mastoparan significantly activated p.001 phosphatidylinositol and phosphatidylinositol-phosphate kinase activities in a time and dose dependent fashion. These data suggest that the cellular toxic effects of mastoparan may in part be due to changes in phospholipid metabolism, thereby altering membrane structure and function.

Subject Categories:

  • Biochemistry
  • Biology
  • Anatomy and Physiology
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE