Accession Number:

ADA634068

Title:

Differential Expression of Inward and Outward Potassium Currents in the Macrophage-like Cell Line J774.1

Descriptive Note:

Doctoral thesis

Corporate Author:

UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD

Personal Author(s):

Report Date:

1985-04-02

Pagination or Media Count:

151.0

Abstract:

The electrophysiological properties of the J774.1 macrophage-like cell line were studied at 25 deg C. Both conventional intracellular microelectrode and whole-cell patch microelectrode techniques were used. Long-term adherent 24 hours cultures investigated with intracellular electrodes exhibited a broad distribution of resting membrane potentials and membrane resistances RMP approximately -50 - 3.0 mV, Rm approximately 117 - 13 Mohm, Mean - SEM, n approximately 55. Most cells with hyperpolarized RMPs exhibited inward rectification.

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE