Etiology and Effects of Ciomiphene on Cystic Endometrial Hyperplasia in the Miniature Pig
UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD
Pagination or Media Count:
Cystic endometrial hyperplasia CEH of unknown etiology has been discovered in Swine Leukocyte Antigen SLA inbred miniature pigs. In humans endometrial hyperplasia is known most often to be associated with hyperestrogenism and thus it was hypothesized that CEH in SLA miniature pigs may also be due to hyperestrogenism. The present investigation was undertaken to perform a histological and biochemical characterization of CEH in miniature pigs. The primary objectives of this study were to 1 document the gross morphological and histological changes of the endometrium associated with CEH, 2 determine whether differences exist in hormonal and or steroid hormone receptor concentrations between CEH and non-CEH animals, 3 determine whether CEH can be induced by unopposed estrogen action and 4 examine the effects of progesterone and clomiphene citrate on the progression of existing CEH condition. The SLA miniature sows were assigned as CEH or non-CEH, based on the examination of the uterus by a midventral laparotomy. When necessary, animals were bilaterally ovariectomized during the laparotomy. Serum concentrations of estrone El, estradiol E2, progester one P, testosterone T and luteinizing hormone LH were measured by specific radioimmunoassays RIA. Uterine tissue samples for histology and for quantitation of receptors were collected from sows at necropsy. Sodium dodecyl sulfate . SDS-polyacrylamide gel electrophoresis was used for the qualitative analysis of proteins in cyst fluid, uterine flushings and serum samples. Endometrial estrogen and progesterone receptors were quantitated by radiolabelled ligand binding assay. Hematoxylin and eosin staining of tissue was utilized for the histological analysis.
- Medicine and Medical Research