Accession Number:

ADA629497

Title:

Red Blood Cells Accelerate the Onset of Clot Formation in Polytrauma and Hemorrhagic Shock

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Report Date:

2010-11-01

Pagination or Media Count:

9.0

Abstract:

Background Hemorrhage and coagulopathy are major contributors to death after trauma. The contribution of red blood cells RBCs in correcting coagulopathy is poorly understood. Current methods of measuring coagulopathy may fail to accurately characterize in vivo clotting. We aimed to determine the effect of RBCs on clotting parameters by comparing resuscitation regimens containing RBCs and plasma with those containing plasma alone. Methods Thirty-two Yorkshire swine were anesthetized, subjected to a complex model of polytrauma and hemorrhagic shock, and resuscitated with either fresh frozen plasma, lyophilized plasma LP, or 11 ratios of fresh frozen plasmapacked RBC PRBC or LPPRBC. Activated clotting time, prothrombin time, partial thromboplastin time, and thrombelastography TEG were performed at 1 hour, 2 hours, 3 hours, and 4 hours after resuscitation. Results Animals treated with 11 LPPRBC had less blood loss than the other groups p less than 0.05. The activated clotting time was shorter in the 11 groups when compared with the pure plasma groups at all time points p less than 0.05. The 11 groups had shorter TEG R times time to onset of clotting at 1 hour, 3 hours, and 4 hours compared with pure plasma groups p less than 0.05. Other TEG parameters did not differ between groups. Partial thromboplastin time was shorter in the pure plasma groups than the 11 groups at all time points p less than 0.05. Conclusions Whole blood assays reveal that RBCs accelerate the onset of clot formation. Coagulation assays u

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE