Accession Number:

ADA628719

Title:

Methicillin-Resistant Staphylococcus aureus in Wound Cultures Recovered from a Combat Support Hospital in Iraq

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Report Date:

2010-07-01

Pagination or Media Count:

8.0

Abstract:

Background Staphylococcus aureus infections complicate care of combatrelated injuries and can independently result in skin and soft-tissue infections during deployments or training. Community-associated methicillin-resistant S. aureus CA-MRSA strains seem to produce severe disease but retain susceptibility to many oral antimicrobials. This study characterizes 84 MRSA isolates recovered from wound cultures at a combat support hospital in Iraq. Methods MRSA strains recovered from December 2007 through March 2009 were analyzed. Antimicrobial resistance testing was determined by broth microdilution and the BD Phoenix Automated Microbiology System. The genotypic pattern was analyzed by pulsed-field gel electrophoresis and polymerase chain reaction identification of resistance and virulence genes. Results No MRSA isolates from wound cultures were resistant to vancomycin. The most active oral antistaphylococcal agents were tetracycline 95 susceptibility, trimethoprim-sulfamethoxazole 94, and clindamycin 94. Of agents not typically recommended as monotherapy, 98 of isolates were susceptible to rifampin, 91 to moxifloxacin, and 60 to levofloxacin. The most common pulsed-field type PFT was USA300 79. The typical staphylococcal cassette chromosome mec IV elements carrying the CA-MRSA resistance genes were present in 88 of the isolates. Panton-Valentine leukocidin virulence genes were identified in 88 of isolates, including 100 of PFT USA300. The virulence gene associated with an arginine catabolic mobile element was present in 75 of isolates, including 94 of PFT USA300.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE