Accession Number:

ADA628331

Title:

Integra as a Dermal Replacement in a Meshed Composite Skin Graft in a Rat Model: A One-Step Operative Procedure

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Report Date:

2002-01-01

Pagination or Media Count:

9.0

Abstract:

Background Current use of Integra, the collagen-based dermal analogue, requires a two-step grafting procedure to achieve wound closure with an ultrathin autograft. Methods A one-step operative procedure of meshed composite skin graft MCSG using Integra as a dermal template for a meshed split thickness autograft was developed in rats. The silicon layer of Integra was removed, the resulting dermal analogue was meshed 11.5, expanded, and placed on excised full thickness wound and covered with a meshed 11.5 or 16 split thickness autograft. Grafted wounds were dressed with BioBrane, Vaseline gauze, silver-impregnated nylon, or silver-nylon and direct current SNDC. At scheduled intervals up to 3 months postgrafting, wounds were examined for epithelialization, collagen deposition and fibrosis, hair growth, and contraction. The results of wound clo- sure and healing following the one-step procedure were compared with the out- come of the two-step grafting procedure where application of meshed Integra step one was followed in 14 days by removal of the silicon layer and application of the meshed autograft step two. Results The one-step procedure applied to meshed autograftIntegra 11.5 11.5 composite graft accelerated wound closure by 6-19 days when compared with the two-step procedure. At 3 months post- grafting, the contraction of the healed wound dressed with SNDC, BioBrane, or Vaseline gauze was reduced by 13-16 following the one-step procedure compared with the two-step procedure p less than 0.05. The one-step procedure allowed the expansion of the autograft layer to 16 while achieving wound healing results similar to grafting with 11.5 meshed autograft layer using the two-step grafting procedure.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Medical Facilities, Equipment and Supplies

Distribution Statement:

APPROVED FOR PUBLIC RELEASE