Accession Number:

ADA627756

Title:

Cytoprotection of Human Endothelial Cells From Menadione Cytotoxicity by Caffeic Acid Phenethyl Ester: The Role of Heme Oxygenase-1

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Report Date:

2008-06-08

Pagination or Media Count:

9.0

Abstract:

Caffeic Acid Phenethyl Ester CAPE, derived from various plant sources, has been shown to ameliorate ischemiareperfusion injury in vivo , and this has been attributed to its ability to reduce oxidative stress. Here we investigated the cytoprotection of CAPE against menadione-induced oxidative stress in human umbilical vein endothelial cells HUVEC to evaluate potential gene expression involvement. CAPE exhibited dose- dependent cytoprotection of HUVEC. A gene screen with microarrays was performed to identify the potential cytoprotective genes induced by CAPE. Heme oxygenase-1 HO-1 was highly upregulated by CAPE and this was confirmed with reverse transcriptase polymerase chain reaction RT-PCR and western blotting. Inhibition of HO-1 activity using the HO-1 inhibitor tin protoporphyrin IX SnPPIX, resulted in loss of cytoprotection. Carbon monoxide, one of HO-1 catabolic products appeared to play a small role in CAPE protection. Caffeic acid, a potential metabolite of CAPE with similar free radical scavenging ability, however, didnt show any cytoprotective effect nor induce HO-1. These findings suggest an important role of HO-1 induction in CAPE cytoprotection against oxidant stress, which may not relate to CAPE structural antioxidant activity nor to its traditional enzymatic activity in decomposing heme but to a yet to be determined activity.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research
  • Toxicology
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE