Biomarkers in the Detection of Prostate Cancer in African Americans
Annual rept. 1 Sep 2014-31 Aug 2015
ALABAMA UNIV IN BIRMINGHAM
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This grant focuses on identifying molecular features of prostatic adenocarcinomas PrCa from African Americans AAs which we hypothesize may differ from the molecular features of PrCas from European Americans EAs. We give priority to characteristics found to be associated with PrCa aggressiveness, because the differential expression of those characteristics may, in part, explain why AAs tend to have more aggressive PrCa than EAs. Genes involved in fatty acid transport and metabolism have emerged as candidate markers for aggressive PrCa subtypes that are prevalent in AAs. We use an ancestry informed approach in which single nucleotide polymorphisms SNPs define more accurately the racial ancestry of our study subjects. AAs with PrCa are more often treated non-surgically and thus fewer radical prostatectomies are available to study PrCa in AAs. To overcome this disparity, this project focuses on the analysis of prostate biopsies. We use nitrocellulose blots tissue prints of prostate biopsies as a source of high quality RNA and DNA to identify molecular biomarkers that differ between AAs and EAs. These include PrCa generated field effects that modify adjacent prostate tissues even when they appear to be histopathologically normal such field effects include epigenetic DNA hypermethylation with silencing of specific genes.
- Anatomy and Physiology
- Medicine and Medical Research