Examination of Urinary Beta-Naphthol as a Biomarker Indicative of Jet Fuel Exposures
Interim rept. Jan 2014-Apr 2015
HENRY M JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE WRIGHT-PATTERSON AFB OH
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A joint US Air ForceJapan Air Self-Defense Force JASDF study was conducted to examine molecular responses in a human cohort occupationally exposed to either JP-4 or JP-8. In this sub-study, we examined the utility of urinary beta-naphthol uBetaNph as a biomarker of exposure to jet fuels. Beta-naphthol is a metabolic product of naphthalene, a poly aromatic hydrocarbon found in jet fuel. Published studies using human occupational exposure samples indicated that urinary increases in BetaNph correlate well with jet fuel exposure levels. This study used a Beta-naphthol competitive enzyme-linked immunosorbent assay ELISA to quantitate urine samples taken from subjects 18-50 yrs old prior to shift or immediately post-shift. Exposed group subjects worked in occupations crew chief, flight line personnel with likely jet fuel exposures, while the control group was matched for location but in occupations with no expected exposure office workers. Both JP-4 and JP-8 exposurecontrol group urine samples were collected and analyzed for BetaNph as well as uBEtaNph - urinary BetaNph normalized with creatinine levels to account for urine volume dilution. Statistical analyses of the meta data and uBetaNph included standard analysis of variance, Mann-Whitney Rank Sum test, factor analysis, dimensionality assessment, and correlation figures. Unlike other studies, we did not see any correlation to exposure group, nor did we see indications that smoking was a confounding factor in our analyses. However, our data suggested that age does correlate with BetaNph levels. Further studies using a larger cohort and accurate quantitation of jet fuel dose should clarify issues seen in this study with the use of uBetaNph as a urinary biomarker of jet fuel exposure.
- Anatomy and Physiology