Accession Number:

ADA626555

Title:

Acute Pancreatitis as a Model to Predict Transition of Systemic Inflammation to Organ Failure in Trauma and Critical Illiness

Descriptive Note:

Annual rept. 22 Sep 2014-21 Sep 2015

Corporate Author:

PITTSBURGH UNIV PA

Report Date:

2015-10-01

Pagination or Media Count:

23.0

Abstract:

Trauma, extensive burns, bacterial infections, and acute pancreatitis AP are common conditions of tissue injury and immune system activation that can result in the systemic inflammatory response syndrome SIRS. Surprisingly, about half of the patients with SIRS quickly recover, while the others develop a multiorgan dysfunction syndrome MODS. SIRS and MODS do not occur immediately SIRS evolves over a 4-12 hour period, while MODS evolves over 12-24 hours. Vascular leak syndrome VLS is a critical component of the transition from SIRS to MODS. Understanding the mechanism by which SIRS triggers VLS and progresses to MODS is critical to correctly model disease course thereby aiding in treatment of patients. In this report, we tested the effect of severe acute pancreatitis patient serum on the viability of human vascular endothelial cells grown in a monolayer. The experiments show the disruption of the endothelial monolayer as an in vitro model of VLS and allow a quantitative measure by evaluating cell viability. The results demonstrate differences in viability between AP patients and normal healthy volunteers.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE