Accession Number:

ADA626547

Title:

Targeting Alpha5 Beta1 Integrin to Prevent Metastatic Breast Cancer Cell Invasion: PhScN Target Site Definition and Plasma Stability

Descriptive Note:

Final rept. 1 Sep 2012-31 Aug 2015

Corporate Author:

MICHIGAN UNIV ANN ARBOR

Personal Author(s):

Report Date:

2015-11-01

Pagination or Media Count:

24.0

Abstract:

It has been suggested that PHSCN inhibits metastatic invasion by forming a covalent, disulfide bond with a cysteine residue on the beta1 alpha-5-beta-1 subunit of alpha5 beta1 alpha-5 beta-1 integrin1. However, these studies were performed with purified alpha-5-beta-1 integrin, which also produces evidence of covalent bond formation with the alpha-5 subunit tandem mass spectroscopy data not shown. Hence, the specificity of the reported interaction between PHSCN and the beta-1 subunit1 is suspect. Moreover, the cysteine rich beta-1 subunit can heterodimerize with 12 distinct alpha integrin subunits2, forming integrins that function in many pathways. In contrast, the alpha-5 subunit interacts uniquely with the beta-1 subunit2 to induce invasion and support adhesion 3-5 thus it is a much more desirable target.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE