DURIP: Electrokinetic Injection and Separation System for Analysis of Protein and Peptide Transport, Adsorption and Kinetics Instrumentation Proposal
Final rept. 1 Aug 2013-31 Jul 2014
CALIFORNIA UNIV SANTA BARBARA
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We requested equipment necessary to build an electrokinetic injection and separation system for the analysis of protein and peptide transport, adsorption and kinetics as well as other biomolecular characterization as prescribed by our current Army contracts. Biomolecular, and more specifically proteomic, analyses are vital for fully understanding biological systems. Under a recent PECASE grant awarded to the PI, we are investigating fundamental protein transport, adsorption, and kinetic interactions using a novel nanofluidics-based platform. Since nanofluidic channels are on the same length scale of both the electric double layer that forms at a solid-liquid interface as well as the biomolecules themselves, we can harness the coupled physics of complex biological fluids in nanofluidic channels towards unique, efficient technology that will potentially allow for a truly new regime of proteomic analysis. Specifically, we bought an instrument that helps us validate our characterization instrument, the equipment needed for our system itself, and a variety of corresponding parts that accompany the equipment in order to allow us to operate with real human samples, with whole blood and in darkfield conditions.
- Electricity and Magnetism