Accession Number:

ADA625279

Title:

Development of Augmented Leukemia/Lymphoma-Specific T-Cell Immunotherapy for Deployment with Haploidentical Hematopoietic Progenitor-Cell Transplant

Descriptive Note:

Annual rept. 15 Apr 2008-14 Apr 2009

Corporate Author:

M D ANDERSON CANCER CENTER HOUSTON TX

Personal Author(s):

Report Date:

2009-05-01

Pagination or Media Count:

43.0

Abstract:

This project develops novel immune-based therapies to target childhood leukemia. By combining gene therapy with T-cell therapy we have developed new biologic agents that specifically recognize CD19 molecule on the cell surface of B-cell acute lymphoblastic leukemia B-ALL. To translate this approach to the clinic we have adapted the Sleeping Beauty SB transposontransposase system to express a CD19-specific chimeric antigen receptor CAR. T cells that have undergone transposition express the CAR and therefore redirected specificity for CD19. To selectively propagate CD19-specific T cells to clinically-meaningful numbers we have developed artificial antigen presenting cells aAPC by expressing CD19 molecule and desired co-stimulatory molecules on K562 cells. Thus, by combining SB transposition and aAPC, we can harness gene therapy and T-cell therapy to develop targeted treatment for B-ALL.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Anatomy and Physiology
  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE