A CRISPR-based MLST Scheme for Understanding the Population Biology and Epidemiology of Salmonella Enterica
Final rept. 1 Sep 2011-31 Aug 2014
PENNSYLVANIA STATE UNIV STATE COLLEGE
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Salmonella enterica subsp. enterica is the most common cause of bacterial foodborne illness in the United States. With Army ARO funds, we have investigated over the past three years whether polymorphic regions called CRISPR can used to differentiate strains of this pathogen for outbreak investigations. We have also been investigating whether CRISPR in Salmonella protects the bacteria against foreign DNA as described in other systems, or whether it has alternative functions. Here, we report that CRISPR can be used to subtype Salmonella enterica serovariants Heidelberg and Typhimurium, and discuss findings from sequence analysis of over 600 strains performed over the course of this study.
- Medicine and Medical Research