Role of APOE Isoforms in the Pathogenesis of TBI Induced Alzheimer's Disease
Annual rept. 30 Sep 2014-29 Sep 2015
PITTSBURGH UNIV PA
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During the reported period, we have been able to generate all experimental groups of mice on E3 and E4 genetic background and have completed the experiments with two age groups 3 and 9 months. Three behavioral paradigms were used to evaluate the differences in cognitive performance between the genotypes and age groups. The analysis of the results two parameters in Morris Water Maze demonstrated that while the young mice sham and injured of both genotypes recover faster, the differences do not reach significance. In the other two paradigm Elevated Plus Maze and Contextual Fear Conditioning there were differences in performance between the genotypes in the old groups only. All brain samples from mice of the above groups have been processed and libraries generated for mRNA-seq. All libraries have been sequenced and the sequencing files transferred from NGS Center at UPenn, processed and analyzed. Differential expression tables showed clearly delineated GO categories of up- and down-regulated genes between sham and injured mice. The results of the study at this stage have been presented at the SfN meeting in Chicago in October, 2015.
- Medicine and Medical Research