Accession Number:

ADA624260

Title:

High-Throughput Sequencing of Germline and Tumor From Men with Early-Onset Metastatic Prostate Cancer

Descriptive Note:

Annual rept. 30 Sep 2014-29 Sep 2015

Corporate Author:

MICHIGAN UNIV ANN ARBOR

Personal Author(s):

Report Date:

2015-10-01

Pagination or Media Count:

11.0

Abstract:

In this project, we proposed to use next generation sequencing approaches to analyze germline DNA and prostate cancer tissue from 20 men with early-onset, metastatic prostate cancer in order to identify variants that increase the risk of developing clinically significant prostate cancer, as well as novel driving somatic alterations. We have successfully, enrolled 18 men and are currently conducting whole exome sequencing on germline DNA from whole blood and comprehensive molecular analyses on formalin-fixed paraffin-embedded prostate tumors from these cases. In the coming year, we plan to complete enrollment, tissue procurement, and sequencing. We will also confirm tumor findings in a larger set of aggressive prostate cancer cases. Given the uniqueness of the patient cohort in this project, we expect that novel driving genes with germline andor somatic variants will be identified, which can be followed up with future functional studies

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE