Mechanisms of Bacterial Spore Germination and Its Heterogeneity
Final rept. 1 Jul 2009-31 Aug 2014
CONNECTICUT UNIV HEALTH CENTER FARMINGTON
Pagination or Media Count:
Great progress has been made in understanding the mechanisms of Bacillus and Clostridium spore germination and its heterogeneity in the 5 years of the MURI award. The following advances have been made 1 high resolution structures of four germination proteins have been determined 2 reasons for the heterogeneity in rates of spore germination have been determined, a major one being variations in levels of germinant receptors GRs between individual spores 3 many factors modulating GR levels in spores have been identified 4 a number of new early events in germination have been identified including acquisition of spore memory of germinant exposure, and a drastic change in spore inner membrane properties 5 GRs in B. subtilis spores were shown to assemble in a single focus in spores inner membrane in a GerD protein dependent manner 6 large numbers of refinements have been made in technology to simultaneously examine the germination of 100s of individual spores 7 high-pressure germination of multiple individual spores has been examined 8 a mathematical model describing spore germination has been developed 9 much of the work above has been extended to Clostridium spores and 10 approximately 90 research papers have or will be published.
- Anatomy and Physiology