Accession Number:

ADA624153

Title:

Identification of Biomarkers Associated with the Healing of Chronic Wounds

Descriptive Note:

Final rept. 15 Jun 2005-31 Dec 2007

Corporate Author:

DAEMEN COLL AMHERST NY

Personal Author(s):

Report Date:

2008-01-01

Pagination or Media Count:

23.0

Abstract:

It is the objective of this study to identify the biomarkers associated with the earliest stages of healing in chronic wounds. The findings of this study are intended to facilitate the development a diagnostic tool, which could be used to evaluate the healing process. Samples have been collected from interior and peripheral wound sites over 6 weeks from 48 subjects thus far and analyzed using 2D Page and iTRAQTM. 700 spots have been recognized using ProteomweaverTM software to analyze the gels and 207 proteins have been identified in wound samples using iTRAQTM. All wounds analyzed with iTRAQTM to date share 84 proteins, 25 proteins were only found in chronic wounds and 31 proteins were only found in healing wounds. Significant p.05 changes in the amount of proteins over the time course of the wound were found in 149 of the 207 proteins. The correlation of wound biochemistry, clinical appearance, and clinical outcome is critical to understanding of pressure ulcer healing. These findings will aid in the development of criteria for evaluating the healing process and response to treatment.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE