Host Genes and Resistance/Sensitivity to Military Priority Pathogens
Annual rept. 1 Jun 2009-31 May 2010
TENNESSEE UNIV MEMPHIS HEALTH SCIENCE CENTER
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The major objective of the award is to identify host genetic locipathways whose expressionlack of expression correlate with resistance vs. susceptibility to pathogenic challenge using recombinant inbred BXD mice. The DoD priority pathogens studied are Francisella tularnsis FT, Burhholderia, Acinetobacter, Leishmania major Lm, SARS, H5N1 avian influenza. The FT project has made progress with the IVS imaging of respiratory infections in mice, and generated and characterized attenuated mutant strains of FT LVS. The Acinetobacter project has generated a luminescence reporter vector, which facilitates in vivo IVIS imaging, and in vitro studies suggest that A. baumanii activates TLR4 pathway. The Leishmania project revealed differences in the immunological and clinical response shown by C57BL6 and DBA2 parental strains to Lm. The influenza project has made progress with generating their congenic mouse lines. The mouse genomics core maintains the BXD mouse colonies and has produced full genome sequencing of the DBA2 parental strains and collected gene expression data from the spleen. The bioinformatics core has developed and integrated the algorithms to model the genetic pathways underlying the host responses to pathogen infections, and tested the algorithm using BXD mice infected with Chlamydia psittaci to provide a model for infection with other DoD priority pathogens.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research