Accession Number:

ADA623663

Title:

An Experimental Investigation of the Performance of a Collison Nebulizer Generating H1N1 Influenza Aerosols

Descriptive Note:

Journal article

Corporate Author:

AIR FORCE CIVIL ENGINEER CENTER (DETACHMENT 1 EAST) TYNDALL AFB FL READINESS DIR

Report Date:

2015-04-04

Pagination or Media Count:

9.0

Abstract:

The performance of a Collison nebulizer delivering viable H1N1 influenza aerosols was assessed in terms of particle size distribution PSD and survivability of the virus upon generation. An H1N1 influenza virus preparation in egg allantoic fluid was diluted in sterile deionized water to a concentration of 3.4 106 TCID50mL. The virus suspension was aerosolized at air flow rates of 2, 6 and 12 Lmin using a 1-jet, 3-jet and 6-jet Collison nebulizer, respectively. A scanning mobility particle sizer measured the PSD of the viral aerosol after steady-state delivery times of 1, 15, 30, 45 and 60 min. After 60 min of continuous aerosolization, the viral titre was unchanged and the count median diameter CMD of the aerosol PSD was 38 nm for the 2 Lmin flow rate, 35 nm for the 6 Lmin flow rate and 33 nm for the 12 Lmin flow rate. The CMDs were much smaller than the influenza virus 80 120 nm, indicating the aerosol distribution comprised mainly nonviable materials. The PSD produced by the Collison nebulizer exhibited a 20 increase in peak particle concentration after 60 min of continuous operation at 12 Lmin. This progressive increase in particle counts may be attributed to a combination of evaporation and shear and impact stresses imparted on components by the Collison nebulizer. The possible slight loss in H1N1 influenza viability over the course of 60 min of continuous aerosolization at 12 Lmin is consistent with previous bioaerosol studies using a Collison nebulizer.

Subject Categories:

  • Microbiology
  • Physical Chemistry
  • Atomic and Molecular Physics and Spectroscopy

Distribution Statement:

APPROVED FOR PUBLIC RELEASE