Accession Number:
ADA623360
Title:
Architecture and Assembly of the Bacillus subtilis Spore Coat
Descriptive Note:
Journal article
Corporate Author:
CONNECTICUT UNIV HEALTH CENTER FARMINGTON
Personal Author(s):
Report Date:
2014-09-26
Pagination or Media Count:
19.0
Abstract:
In this study, atomic force microscopy was used to probe the coat structure of Bacillus subtilis wild-type and cotA, cotB, safA, cotH, cotO, cotE, gerE and cotE gerE spores. This approach provided high-resolution visualization of the various coat structures, new insight into the function of specific coat proteins, enabled the development of a detailed model of spore coat architecture, and identified several spore coat layers that have not previously been observed. This work also provided evidence that the spores inner coat is crystalline, with its apparent two-dimensional 2D nuclei being the first example of a non-mineral 2D crystallization pattern in a biological organism.
Descriptors:
- *ASSEMBLY
- *BACILLUS SUBTILIS
- *SPORES
- AMORPHOUS MATERIALS
- ARCHITECTURE
- ATOMIC SPECTROSCOPY
- BACTERIA
- BIOLOGICAL DETECTION
- CELLS(BIOLOGY)
- COATINGS
- CRYSTALLIZATION
- ELECTRON MICROSCOPY
- ENZYMES
- FIBERS
- GERMINATION
- HIGH RESOLUTION
- HONEYCOMB STRUCTURES
- MOLECULAR DYNAMICS
- NANOPARTICLES
- NUCLEATION
- PATTERNS
- PROTEINS
- STRAINS(BIOLOGY)
Subject Categories:
- Biochemistry
- Microbiology
- Crystallography
- Atomic and Molecular Physics and Spectroscopy