Accession Number:

ADA623185

Title:

Renewable Bio-Solar Hydrogen Production: The Second Generation (Part B)

Descriptive Note:

Final rept. 15 May 2011-31 Dec 2014

Corporate Author:

PENNSYLVANIA STATE UNIV STATE COLLEGE DEPT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY

Personal Author(s):

Report Date:

2015-03-20

Pagination or Media Count:

13.0

Abstract:

In this project we used targeted mutagenesis, overexpression of genes, transcription profiling, and metabolic profiling of the cyanobacterium Synechococcus sp. PCC 7002 to understand better how to optimize the production of biohydrogen and potential carbon-based biofuel molecules. A major finding was that the tricarboxylic acid TCA cycle is not branched, as had been believed for nearly 50 years, but is in fact closed by two noncanonical enzymes that replace 2-oxoglutarate dehydrogenase. In addition to redefining the TCA cycle, we validated the occurrence of the glyoxylate cycle and the gamma-aminobutyric acid GABA shunt in a few cyanobacteria, and we studied the partitioning of metabolites between the glycolytic and oxidative pentose phosphate pathways. Important transcription regulators, including RbcR, Fur, and ChlR, were identified and characterized. Approaches to maximize biohydrogen production and carbon-based biofuels e.g., cellulose, poly-hydroxybutyrate were characterized and optimized. These studies yielded important new biochemical details about critically important aspects of cyanobacterial physiology and metabolism.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE