Development of a Chronic Toxicity Testing Method for Daphnia pulex
ENGINEER RESEARCH AND DEVELOPMENT CENTER VICKSBURG MS ENVIRONMENTAL LAB
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Acquiring reliable toxicological data about cladocerans is important for gauging any environmental contaminant of concern. Acute and chronic bioassay methods for the testing of cladocerans water fleas were developed by the American Society for Testing and Materials ASTM, United States Environmental Protection Agency USEPA, Organisation for Economic Cooperation and Development OECD, and International Organization for Standardization ISO and have been extensively employed to determine the hazard of many substances. These organizations currently do not provide a standard chronic toxicity test method for assessing environmental contaminants to the cladoceran Daphnia pulex. This organism is important for toxicological testing as it is very sensitive to contaminants and has a well-sequenced genome for toxicogenomics investigations. Since a chronic D. pulex method was required to execute research conducted at the U.S. Army Engineer Research and Development Center s ERDC Environmental Laboratory, modifications were made to the current USEPA 2002 chronic method for Ceriodaphnia dubia and the ASTM 2012 and OECD 2008 chronic methods for Daphnia magna. The protocol described in this technical report provides a step-by-step chronic toxicity method specifically for Daphnia pulex, with the following adaptations 1 modifications to the exposure chambers and water exchange apparatus and methods 2 modifications to the organism loading procedure and 3 modifications to the test duration to accommodate 3-broods. The protocol yields reproducible toxicological endpoint data.
- Genetic Engineering and Molecular Biology
- Test Facilities, Equipment and Methods