The Role of NFIB in Prostate Cancer Progression
Annual rept. 1 Sep 2014-31 Aug 2015
VANDERBILT UNIV NASHVILLE TN
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Nuclear factor IB NFIB is a co-factor of androgen receptor AR and FOXA1. Previous studies in the laboratory have suggested a role for NFIB in controlling neuroendocrine differentiation loss of AR expression, gain of neuroendocrine markers like synaptophoysin during prostate cancer progression. Knockdown of NFIB expression in LNCaP and C4-2B prostate cancer cell lines results in increased AR message and protein levels, but synaptophysin levels were not affected. 22RV1 cells, which are not reported to undergo neuroendocrine differentiation, expressed synaptophysin and AR at an increased level in response to NFIB over-expression. 22RV1-NFIB cells were also more proliferative than their 22RV1-vector controls in both serum-containing and serum-free media. These studies are defining a role for NFIB regulation of AR and synaptophysin in prostate cancer in vitro and suggest a role for NFIB during prostate cancer progression in vivo.
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