Accession Number:

ADA622595

Title:

Brain Transcriptome Profiles in Mouse Model Simulating Features of Post-traumatic Stress Disorder

Descriptive Note:

Journal article

Corporate Author:

ARMY CENTER FOR ENVIRONMENTAL HEALTH RESEARCH FORT DETRICK MD

Report Date:

2015-02-28

Pagination or Media Count:

23.0

Abstract:

Social-stress mouse model, based on the resident-intruder paradigm was used to simulate features of human post-traumatic stress disorder PTSD. The model involved exposure of an intruder subjectmouse to a resident aggressor mouse followed by exposure to trauma reminders with rest periods. C57BL6 mice exposed to SJL aggressor mice exhibited behaviors suggested as PTSD-in-mouse phenotypes intermittent freezing, reduced locomotion, avoidance of the aggressor-associated cue and apparent startled jumping. Brain tissues amygdala,hippocampus, medial prefrontal cortex, septal region, corpus striatum and ventral striatum from subject aggressor exposed Agg-E and control C57BL6 mice were collected at one, 10 and 42 days post aggressor exposure sessions. Transcripts in these brain regions were assayed using Agilents mouse genome-wide arrays. Results Pathways and biological processes associated with differentially regulated genes were mainly those thought to be involved in fear-related behavioral responses and neuronal signaling. Expression-based assessments of activation patterns showed increased activations of pathways related to anxiety disorders hyperactivity and fear responses, impaired cognition, mood disorders, circadian rhythm disruption, and impaired territorial and aggressive behaviors. In amygdala, activations of these pathways were more pronounced at earlier time-points, with some attenuation after longer rest periods. In hippocampus and medial prefrontal cortex, activation patterns were observed at later time points. Signaling pathways associated with PTSD-comorbid conditions, such as diabetes, metabolic disorder, inflammation and cardiac infarction, were also significantly enriched. In contrast, signaling processes related to neurogenesis and synaptic plasticity were inhibited.

Subject Categories:

  • Psychology
  • Biochemistry
  • Medicine and Medical Research
  • Stress Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE