Development of Advanced Technologies for Complete Genomic and Proteomic Characterization of Quantized Human Tumor Cells
Final rept. 15 June 2011 - 14 June 2015
SWEDISH HEALTH SERVICES SEATTLE WA
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This research proposal provides important insights into cancer mechanisms and blood biomarkers to assess progression and stratification of human glioblastoma. We have successfully established several patient-derived cell lines from glioblastoma tumors and further established a number of quantized cell populations from these parental tumor cell lines. The cell populations have been used for extensive molecular characterization, including whole genome sequencing, transcriptomic analysis, proteomics secretome as well as N-glycocapture analysis, and high-throughput drug screening for evaluating the effectiveness of drug candidates in targeting these cell populations. Combined, this program significantly advances genomic, proteomic and single-cell technologies. Although the focus of this research is glioblastoma, the proposed tools are generally applicable to all cancer-based studies.
- Medicine and Medical Research