Accession Number:

ADA622384

Title:

Development of Nanomedicines for Treatment of Posttraumatic Osteoarthritis

Descriptive Note:

Annual rept. 5 Jun 2014-4 Jun 2015

Corporate Author:

NEBRASKA UNIV MEDICAL CENTER OMAHA

Report Date:

2015-07-01

Pagination or Media Count:

8.0

Abstract:

During this funding period, N-2-hydroxypropylmethacrylamide HPMA copolymers dexamethasone conjugates were synthesized and labeled with near-infrared dye IRDye CW 800 or Alexa Fluor 647 P-Dex-IRDye or P-Dex-Alexa. Mice with surgical destabilization of the medial meniscus DMM were established as an osteoarthritis model for our evaluation. P-Dex-IRDye and P-Dex- Alexa were given to the DMM mice via intraarticular injection. Optical imaging data suggest that the DMM joints were able to retain the injected P-Dex-IRDye. The treated joints have been isolated for immunohistochemistry and flow cytometry analysis to identify cell phenotypes that internalize the P-Dex-Alexa.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE