Confocal Raman Microspectroscopy: The Measurement of VX Depth Profiles in Hairless Guinea Pig Skin and the Evaluation of RSDL
Technical rept. Aug 2011-Aug 2012
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
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VX depth profiles were obtained using a confocal Raman microspectrometer River Diagnostics with a specially designed microscope objective to focus low power laser light into the skin. Noninvasively and in real-time, the scattered light was collected to a depth of about 100 m with an axial resolution of approximately 4 m. The objectives of this study were to use confocal Raman microspectroscopy to investigate the fate of percutaneously applied VX in the upper skin layers of hairless guinea pigs and to determine the ability of Reactive Skin Decontamination Lotion RSDL to remove or degrade VX from the skin surface and from any depots forming below the skin surface. VX was observed to remain in the stratum corneum, 0-20 m deep, at about 10-20 minutes postexposure. This postexposure VX concentration in this stratum corneum depot decreased with time but was still detectable at 48 hr postexposure. RSDL was observed to remove or degrade VX from both the skin surface and the depot below the skin surface. Our conclusions are that confocal Raman microspectroscopy is an effective tool to obtain real-time, non-invasive analytical data on the concentration of chemicals in the upper skin layers and that RSDL effectively removes or degrades VX from the skin surface and from the depot formed in the upper skin layers following percutaneous exposure.
- Anatomy and Physiology
- Chemical, Biological and Radiological Warfare
- Atomic and Molecular Physics and Spectroscopy