Accession Number:

ADA621292

Title:

Targeting Palmitoyl Acyltransferases in Mutant NRAS-Driven Melanoma

Descriptive Note:

Annual rept. 1 Aug 2013-31 Jul 2014

Corporate Author:

MASSACHUSETTS GENERAL HOSPITAL BOSTON

Personal Author(s):

• Wu, Xu

Report Date:

2014-08-01

Pagination or Media Count:

19.0

Abstract:

Mutations in N-RAS are linked to approximately 20 of melanoma with no effective treatment. Targeting palmitoyl acyltansferases PATs involved in N-RAS regulation could be a novel strategy to treat N-RAS mutant melanoma. The objective of the project is to identify PATs responsible for NRAS activation in melanoma cells using chemical biology and functional genomic approaches. In the first year of the study, we have developed more potent chemical probes to profile PATs in cells, and have carried out PATs profiling in melanoma cells using chemical probes and mRNA profiling. We have identified candidate PATs highly expressed in NRAS melanoma cells. We have developed shRNA reagents to further study their functions.

Descriptors:

• *CANCER
• *NEUROBLASTOMA
• *PROTEOMICS
• ANTIBODIES
• APOPTOSIS
• BIOPHYSICS
• CELLS(BIOLOGY)
• CLINICAL MEDICINE
• DATA MINING
• ENZYMES
• GENE EXPRESSION
• GENOME
• INHIBITION
• MOLECULAR DYNAMICS
• MUTATIONS
• NEOPLASMS
• NEUROLOGY
• PROTEINS
• RECEPTOR SITES(PHYSIOLOGY)
• RESPONSE(BIOLOGY)
• RIBONUCLEIC ACIDS
• SIGNAL PROCESSING
• SYNTHESIS(CHEMISTRY)
• TISSUES(BIOLOGY)

Subject Categories:

• Biochemistry
• Genetic Engineering and Molecular Biology
• Anatomy and Physiology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE