Accession Number:

ADA620464

Title:

Gamma Delta T-Cells Regulate Inflammatory Cell Infiltration of the Lung after Trauma-Hemorrhage

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Report Date:

2015-06-01

Pagination or Media Count:

10.0

Abstract:

CT Trauma-hemorrhage TH promotes acute lung injury ALI and other pulmonary-related complications in part through an exaggerated inflammatory response. Studies have implicated gamma delta T cells in the development of inflammatory complications after major injury however, it is unknown whether gamma delta T cells play a role in the development of ALI after TH. To study this, C57BL6 wild-type WT and delta TCR mice were subjected to TH or sham treatment. Lung injury was clearly evident at 2 h after TH, as evidenced by increased lung permeability, myeloperoxidase levels, and proinflammatory cytokinechemokine levels interleukin-1 beta IL-1 beta , IL-6, IL-10, keratinocyte chemokine, macrophage inflammatory protein 1 beta, macrophage inflammatory protein 1 alpha , and regulated upon activation normal T-cell expressed, secreted chemokine. Phenotypic analysis of lung cells showed an increase in T-cell numbers after TH. The vast majority of these cells were alpha beta T cells, irrespective of injury. Although gamma delta T cells were a small percentage of the total T-cell infiltrate, their numbers did increase after injury. In mice lacking gamma delta T cells delta TCR mice, TH-induced T-cell infiltration of the lung was markedly attenuated, whereas infiltration of other inflammatory cells was increased i.e., monocytes, granulocytes, and myeloid- derived suppressor cells. In conclusion, these findings suggest that gamma delta T cells regulated the infiltration of the lung with inflammatory cells after injury.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE