Accession Number:

ADA620306

Title:

Effect of Calcium Phosphate Coating and rhBMP-2 on Bone Regeneration in Rabbit Calvaria Using Poly(propylene fumarate) Scaffolds

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Report Date:

2015-01-07

Pagination or Media Count:

13.0

Abstract:

Various calcium phosphate based coatings have been evaluated for better bony integration of metallic implants and are currently being investigated to improve the surface bioactivity of polymeric scaffolds. The aim of this study was to evaluate the role of calcium phosphate coating and simultaneous delivery of recombinant human bone morphogenetic protein-2 rhBMP-2 on the in vivo bone regeneration capacity of biodegradable, porous polypropylene fumarate PPF scaffolds. PPF scaffolds were coated with three different calcium phosphate formulations magnesium-substituted b -tricalcium phosphate b -TCMP, carbonated hydroxyapatite synthetic bone mineral, SBM and biphasic calcium phosphate BCP. In vivo bone regeneration was evaluated by implantation of scaffolds in a critical-sized rabbit calvarial defect loaded with different doses of rhBMP-2. Our data demonstrated that scaffolds with each of the calcium phosphate coatings were capable of sustaining rhBMP-2 release and retained an open porous structure. After 6 weeks of implantation, micro-computed tomography revealed that the rhBMP-2 dose had a significant effect on bone formation within the scaffolds and that the SBM-coated scaffolds regenerated significantly greater bone than BCP-coated scaffolds. Mechanical testing of the defects also indicated restoration of strength in the SBM and b -TCMP with rhBMP-2 delivery. Histology results demonstrated bone growth immediately adjacent to the scaffold surface, indicating good osteointegration and osteoconductivity for coated scaffolds. The results obtained in this study suggest that the coated scaffold platform demonstrated a synergistic effect between calcium phosphate coatings and rhBMP-2 delivery and may provide a promising platform for the functional restoration of large bone defects.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Biomedical Instrumentation and Bioengineering

Distribution Statement:

APPROVED FOR PUBLIC RELEASE