Interaction of Jet Fuel Hydrocarbon Components with Red Blood Cells and Hemoglobin
Interim rept. Aug 2013-Jun 2014
AIR FORCE RESEARCH LAB WRIGHT PATTERSON AFB OH HUMAN PERFORMANCE WING (711TH) HUMAN EFFECTIVENESS DIR/ BIOEFFECTS DIVISION
Pagination or Media Count:
This study examined the impact of five jet fuel hydrocarbon components on red blood cells RBCs. We examined the biochemical changes to RBCs by measuring mean corpuscular volume MCV, mean corpuscular hemoglobin MCH, and red cell distribution width RDW. Exposed cells were imaged using scanning electron microscopy SEM to verify morphological changes. The induction of oxidative stress was examined using glutathione GSH depletion as a marker of reactive oxygen species. Finally, microscale thermophoresis MST was used to determine the binding interactions between human hemoglobin and the test set of hydrocarbon compounds. For some chemicals, MCV toluene, decane, RDW toluene, octane, ethylbenzene, and MCH ethylbenzene values were sensitive to exposure incubation temperatures room temperature versus 37 oC. SEMimaging indicated formation of 1 crenated red blood cells in all lower dose exposure sets. Dose dependent oxidative stress was seen for all chemical exposures with the exception of high concentrations of tetradecane and toluene. MST revealed binding affinities between purified human hemoglobin monomer and the hydrocarbons decane KD 2.4 micro M, tetradecane KD 8.8 micro M, and octane KD 5.6 micro M, with toluene demonstrating the tightest binding to hemoglobin at KD 1.9 micro M. Collectively, the apparent increase in the surface area of the cell membrane, GSH depletion, and interaction between the hydrocarbon and hemoglobin molecule may contribute to potential toxicity of these chemicals causing adverse effects on hemodynamics and circulatory function.
- Anatomy and Physiology