Identification and Transcription Profiling of NDUFS8 in Aedes taeniorhynchus (Diptera: Culicidae): Developmental Regulation and Environmental Response
AGRICULTURAL RESEARCH SERVICE GAINESVILLE FL CENTER FOR MEDICAL AGRICULTURAL AND VETERINARY ENTOMOLOGY
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The cDNA of a NADH dehydrogenase-ubiquinone Fe-S protein 8 subunit NDUFS8 gene from Aedes Ochlerotatus taeniorhynchus Wiedemann has been cloned and sequenced. The 824 bp full-length mRNA sequence of AetNDUFS8 encodes an open reading region of 651 bp that is, 217 amino acids. To identify if AetNDUFS8 is developmentally regulated, we employed a quantitative real-time polymerase chain reaction to examine AetNDUFS8 gene expression levels in all developmental stages of Ae. taeniorhynchus. In egg, larval, and pupal stages, AetNDUFS8 was expressed at relatively low levels. However, in the teneral adult stage, AetNDUFS8 was highly expressed. During the time course study in response to permethrin pesticide treatment, quantitative real-time polymerase chain reaction PCR also showed that mRNA transcription levels of AetNDUFS8 were regulated in female Ae. taeniorhynchus. A mitochondrially encoded NADH dehydrogenase subunit 5 AetNADH5 was highly expressed in different developmental stages of Ae. taeniorhynchus. This study suggests that AetNDUFS8 and AetNADH5 play an essential role in the development of Ae. taeniorhynchus and will provide information useful for developing dsRNA pesticide for mosquito control.
- Agricultural Chemistry
- Genetic Engineering and Molecular Biology