Dual Infection of Novel Influenza Viruses A/H1N1 and A/H3N2 in a Cluster of Cambodian Patients
NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA
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During the early months of 2009, a novel influenza AH1N1 virus pH1N1 emerged in Mexico and quickly spread across the globe. In October 2009, a 23-year-old male lawyer residing in central Cambodia was diagnosed with pH1N1. Subsequently, a cluster of four influenza-like illness cases developed involving three children who resided in his home, and the childrens school teacher. Base composition analysis of internal genes using reverse transcriptase polymerase chain reaction and electrospray ionization mass spectrometry revealed that specimens from two of the secondary victims were coinfected with influenza AH3N2 and pH1N1. Phylogenetic analysis of the hemagglutinin genes from these isolated viruses demonstrated that they were closely related to existing pH1N1 and AH3N2 viruses circulating in the region. Genetic recombination was not evident within plaque-purified viral isolates upon full genome sequencing. This incident confirms dual influenza virus infections and highlights the risk of zoonotic and seasonal influenza viruses to coinfect and possibly reassort where they cocirculate.
- Medicine and Medical Research