Development and Resuscitation of a Sedated, Mature Male Miniature Swine Severe Hemorrhage Model
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
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Background A sedated, mature male miniature swine hemorrhage model has been specifically developed to evaluate resuscitation products for the Defense Advanced Research Projects Agency Surviving Blood Loss program. Methods Animals were placed in a sling, sedated with midazolam, and hemorrhaged 60 of estimated blood volume 39 mLkg exponentially for 1 hour with no resuscitation control n 16. An additional 26 swine were treated similarly, then resuscitated with 1 mLkgmin of Hextend to a systolic blood pressure of either 65 mm Hg - 2 mm Hg n 7 or 80 mm Hg - 5 mm Hg n 7 and with 17 beta-estradiol E2 at 1 mgkg n 6 or 10 mgkg n 6. Animals were observed for 3 hours with periodic blood sampling. Survival times for the two E2 groups were not significantly different p 0.59 therefore, the groups were combined for comparison with control. Results Hemorrhage resulted in a characteristic hypotension and metabolic acidosis. Survival time for the control swine was 64 minutes - 11.5 minutes with a 6 survival at 180 minutes. The 180 minutes Hextend survival was 86 for 65 mm Hg and 100 for 80 mm Hg. E2 survival was 125 minutes - 15.3 minutes, significantly different from control p 0.01, but E2 survival of 25 at 180 minutes was not different from control. Conclusion A sedated, sexually mature male miniature swine severe hemorrhage model has been successfully developed, resuscitated with Hextend and used to evaluate E2 as a small volume resuscitation product.
- Anatomy and Physiology
- Medicine and Medical Research